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genechip scanner 3000 7g microarray analysis system  (Thermo Fisher)


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    Thermo Fisher genechip scanner 3000 7g microarray analysis system
    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the <t>microarray</t> results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.
    Genechip Scanner 3000 7g Microarray Analysis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+scanner+3000+7g+microarray+analysis+system/genechip+scanner+3000+7g+microarray+analysis+system/pmc11216254-142-29-26
    Average 90 stars, based on 1 article reviews
    genechip scanner 3000 7g microarray analysis system - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface"

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.65.6.37

    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.
    Figure Legend Snippet: Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Techniques Used: Quantitative RT-PCR, Binding Assay, Expressing, Microarray

    Related Articles

    Microarray:

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface
    Article Snippet: Total RNA of isolated epithelial cells was first isolated using the RNeasy Plus Mini Kit (Qiagen, Inc., Valencia, CA, USA) or TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and then purified using the NucleoSpin RNA Clean-up XS (Macherey-Nagel GmbH & Co. KG, Dueren, Germany) kit. .. Microarray experiments were then performed according to the protocols provided by Thermo Fisher Scientific, and scanned microarray images were obtained using the GeneChip Scanner 3000 7G (Thermo Fisher Scientific) microarray analysis system. .. Isolated total RNA was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany).



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    Thermo Fisher genechip scanner 3000 7g microarray analysis system
    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the <t>microarray</t> results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.
    Genechip Scanner 3000 7g Microarray Analysis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+scanner+3000+7g+microarray+analysis+system/genechip+scanner+3000+7g+microarray+analysis+system/pmc11216254-142-29-26
    Average 90 stars, based on 1 article reviews
    genechip scanner 3000 7g microarray analysis system - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

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    Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Alteration of Gene Expression in Pathological Keratinization of the Ocular Surface

    doi: 10.1167/iovs.65.6.37

    Figure Lengend Snippet: Graphs showing the representative transcripts of genes related to vitamin A metabolism in qRT-PCR. QRT-PCR was also performed for the genes AKR1B15, RDH12, AKR1B10, and CRABP2, which are upregulated more than twentyfold among the significantly upregulated genes and promote binding to RAR, two genes (RARB and RARRES3) whose expression was downregulated more than 1/50, and RARA and RARG, which are other types of RAR. By normalizing the expression of the gene of interest to the expression of 18S rRNA, we were able to obtain a relative measure of the expression level of each gene (* P < 0.05, ** P < 0.005, *** P < 0.0005). Similar to the microarray results, RARB, CRABP2, and RARRES3 showed significant differences, whereas RARA and RARG showed no significant differences.

    Article Snippet: Microarray experiments were then performed according to the protocols provided by Thermo Fisher Scientific, and scanned microarray images were obtained using the GeneChip Scanner 3000 7G (Thermo Fisher Scientific) microarray analysis system.

    Techniques: Quantitative RT-PCR, Binding Assay, Expressing, Microarray